Tunica Vaginalis
This is a serous membrane derived from the peritoneum. It has two sub-layers:
- Parietal Layer: Lines the inner scrotal wall.
- Visceral Layer: Closely adheres to the testis, epididymis, and lower part of the spermatic cord.
- Note: There is a small amount of lubricating fluid between these layers to allow the testis to move freely.
Tunica Albuginea
This is a dense, white fibrous capsule located immediately deep to the tunica vaginalis.
- Function: It is the “skeleton” of the testis. It penetrates the organ to form septa, which divide the testis into approximately 250 compartments called testicular lobules.
Tunica Vasculosa
The innermost layer, consisting of a network of blood vessels supported by delicate connective tissue. It lines the inner surface of the tunica albuginea and the septa.

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